My project employed the power of science to help save the declining honey bee population. I conducted a research project entitled “The Effects of Pesticides, Caffeine & Tea Polyphenols on the Visual and Olfactory Learning and Memory of the Honey Bee,” which explored how to reduce the harmful effects of miticides.
Bees are an essential part of the ecosystem, accounting for one-third of food production and billions of dollars in the global economy. However, there has been a severe decline in the honey bee population. In fact, the global population has declined 40.7% since 2018. This is partly due to a loss of learning and memory skills in the honey bee, caused by harmful pesticides intended to kill bee parasites.
In order to address this problem, I designed a two-part experiment to test whether compounds found in tea and coffee could counter the pesticides’ effects. The spatial experiments tested learning and memory in a setting where the bee was allowed to fly free. The Proboscis Extension Reflex, or PER, experiments tested visual and olfactory learning and memory in a setting where the bees were restricted in a harness.
My key findings were that tea polyphenols and caffeine were able to repair and even improve the bees’ learning and memory after exposure to pesticides. Overall, the visual outperformed the olfactory groups, but tea polyphenols and caffeine were found to be more effective in improving olfactory learning and memory in comparison to visual.
These findings are crucial because beekeepers can add tea polyphenols and caffeine to their sucrose, which could save millions of bees. My research effectively countered the harmful effects of miticides, thus potentially repairing the bees’ learning and memory and saving not only their lives, but also the beekeeping industry and the ecosystem as a whole.
Bees are an essential part of the ecosystem, accounting for one-third of food production and billions of dollars in the global economy. However, there has been a severe decline in the honey bee population. In fact, the global population has declined 40.7% since 2018. This is partly due to a loss of learning and memory skills in the honey bee, caused by harmful pesticides intended to kill bee parasites. My research used tea polyphenols and caffeine to counter the pesticides’ effects, repairing the bees’ learning and memory and saving millions of their lives, the beekeeping industry, and the ecosystem.
Previous research has been conducted on the adverse effects of pesticides and the beneficial effects of tea polyphenols (TP) and caffeine (CA) on honey bee olfactory learning and memory. However, few have tested first, if TP and CA can combat impairment from pesticides; second, the effects of pesticides, TP, and CA on visual and spatial learning and memory; and third, whether bees learn quicker with sight or smell. In order to research these topics, I applied a spatial method and the proboscis extension reflex (PER) procedure to study olfactory learning and memory, as well as adapted the PER method to a visual setting to create an entirely new procedure. During the PER experiments, I created a method to harness bees that is secure and easy to undo when the bees are released, and more efficient than the method used in previous research. My research results show that TP and CA both improved learning and memory after impairment by pesticides, most of the time making it better than control. TP and CA significantly improved bees’ learning by 67.61% and 48.57% respectively in comparison to the control group, and acaricide thymol significantly impaired learning by 54.29%. Additionally, I found that overall visual outperformed olfactory learning and memory, while TP and CA improved olfactory more than visual learning. My novel findings suggest that TP and CA can be used by beekeepers on their honey bees, directly impacting the lives of bees, the beekeeping industry, and the global ecosystem.
For Synopsys, my regional science fair, last year, I recorded a video summarizing my project. It contains clips from my experimentation and bees extending their proboscis live! I talk about my basic methodology as well as describe my different setups.
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